ROCK Inhibition Promotes Attachment, Proliferation, and Wound Closure in Human Embryonic Stem Cell–Derived Retinal Pigmented Epithelium
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چکیده
منابع مشابه
ROCK Inhibition Promotes Attachment, Proliferation, and Wound Closure in Human Embryonic Stem Cell–Derived Retinal Pigmented Epithelium
PURPOSE Nonexudative (dry) age-related macular degeneration (AMD), a leading cause of blindness in the elderly, is associated with the loss of retinal pigmented epithelium (RPE) cells and the development of geographic atrophy, which are areas devoid of RPE cells and photoreceptors. One possible treatment option would be to stimulate RPE attachment and proliferation to replace dying/dysfunctiona...
متن کاملRetinal pigment epithelium wound closure in vitro. Pharmacologic inhibition.
Early-passage bovine retinal pigment epithelium (RPE) cells were grown to confluence in 24-well plates, and a central defect was created mechanically in the monolayer within quadruplicate wells, sequentially over 9 days. Closure of the wounded area occurred by single-cell migration of elongated RPE cells from the edge of the wound and subsequent cell proliferation. Ten days after wounding, the ...
متن کاملCanonical/β-catenin Wnt pathway activation improves retinal pigmented epithelium derivation from human embryonic stem cells.
PURPOSE The purpose of this study was to better understand the role canonical/β-catenin Wnt signaling plays in the differentiation of human embryonic stem cells (hESCs) into retinal pigmented epithelium (RPE), with the goal of improving methods for derivation. METHODS Fluorescent reporters were generated to monitor RPE differentiating from hESCs by using a previously described 14-day derivati...
متن کاملNanotechnology and Regenerative Medicine Canonical/b-Catenin Wnt Pathway Activation Improves Retinal Pigmented Epithelium Derivation From Human Embryonic Stem Cells
Center for Stem Cell Biology and Engineering, University of California, Santa Barbara, California, United States Neuroscience Research Institute, University of California, Santa Barbara, California, United States Department of Molecular, Cellular & Developmental Biology, University of California, Santa Barbara, California, United States California State University, Channel Islands, California, ...
متن کاملDevelopment of a new tissue injector for subretinal transplantation of human embryonic stem cell derived retinal pigmented epithelium
Background Subretinal cell transplantation is a challenging surgical maneuver. This paper describes the preliminary findings of a new tissue injector for subretinal implantation of an ultrathin non-absorbable substrate seeded with human embryonic stem cell-derived retinal pigment epithelium (hESC-RPE). Methods Ultrathin Parylene-C substrates measuring 3.5 mm × 6.0 mm seeded with hESC-RPE (imp...
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ژورنال
عنوان ژورنال: Translational Vision Science & Technology
سال: 2016
ISSN: 2164-2591
DOI: 10.1167/tvst.5.6.7